Hotspot

trnE-UUC-trnT-GGU

LSC · 40513-41316 · 804 bp

Back to Coursetia

Pi 0.318

MarkerSeek score 83.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 240
pi: 0.318
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	ATCAGCAAATTTTTGGGCCG	GAACCGATGACTTACGCCTT	45.0	50.0	57.9	58.0	1089 bp	0.5	59.2
trnE-UUC-trnT-GGU_p2	ATCAGCAAATTTTTGGGCCG	CATCGGAAATATCGGCGGAT	45.0	50.0	57.9	58.0	1566 bp	0.5	59.1
trnE-UUC-trnT-GGU_p3	CCGTCATGTTGGGAGAACAA	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1156 bp	0.5	59.1
trnE-UUC-trnT-GGU_p4	CCGTCATGTTGGGAGAACAA	CATCGGAAATATCGGCGGAT	50.0	50.0	58.1	58.0	1633 bp	0.5	59.0
trnE-UUC-trnT-GGU_p5	ATCAGCAAATTTTTGGGCCG	CCATGGCGTTACTCTACCAC	45.0	55.0	57.9	58.1	1068 bp	0.5	58.7