Hotspot

rps3

LSC · 88275-88931 · 657 bp

Back to Coulteria

Pi 0.00152

MarkerSeek score 59.60

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 1
pi: 0.00152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TCGATATTAAAGATAAGGCAAAGCT	TGAAGAAGAATATGAAAGACGCA	32.0	34.8	56.7	56.3	802 bp	1.0	40.1
rps3_p2	TCGATATTAAAGATAAGGCAAAGC	TGAAGAAGAATATGAAAGACGCA	33.3	34.8	55.4	56.3	802 bp	1.0	40.1
rps3_p3	CGATATTAAAGATAAGGCAAAGCT	TGAAGAAGAATATGAAAGACGCA	33.3	34.8	55.4	56.3	801 bp	1.0	40.1
rps3_p4	TCGATATTAAAGATAAGGCAAAGCT	TGAAGAAGAATATGAAAGACGCAA	32.0	33.3	56.7	57.0	802 bp	1.0	40.1
rps3_p5	TCGATATTAAAGATAAGGCAAAGCT	TTGAAGAAGAATATGAAAGACGCA	32.0	33.3	56.7	57.0	803 bp	1.0	40.1