Hotspot

ndhG-ndhI

SSC · 124487-124911 · 425 bp

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Pi 0.00471

MarkerSeek score 57.80

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 425
variable_sites: 2
pi: 0.00471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAACGAAAAGGCAGAAA	AATCAAATTGCTTTGGGCCG	45.0	45.0	57.9	57.9	620 bp	1.0	79.0
ndhG-ndhI_p2	TCCCAACGAAAAGGCAGAAA	TTTGGGCCGTTTACCAATGT	45.0	45.0	57.9	58.3	609 bp	1.0	77.4
ndhG-ndhI_p3	TCCCAACGAAAAGGCAGAAA	CTTTGGGCCGTTTACCAATG	45.0	50.0	57.9	57.6	610 bp	1.0	76.9
ndhG-ndhI_p4	TCCCAACGAAAAGGCAGAAA	CAAATTGCTTTGGGCCGTTT	45.0	45.0	57.9	58.4	617 bp	1.0	76.5
ndhG-ndhI_p5	TCCCAACGAAAAGGCAGAAA	AATTGCTTTGGGCCGTTTAC	45.0	45.0	57.9	57.6	615 bp	1.0	76.2