Hotspot

rps3-rps19

LSC · 88932-89716 · 785 bp

Back to Coulteria

Pi 0

MarkerSeek score 61.00

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 785
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	TTAAACTTCCGGGGACATGC	50.0	50.0	57.5	58.2	873-896 bp	1.0	76.7
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	TTAAACTTCCGGGGACATGC	50.0	50.0	58.6	58.2	867-890 bp	1.0	75.8
rps3-rps19_p3	TGGTTGTGCAAACCAAAGAG	TTAAACTTCCGGGGACATGC	45.0	50.0	57.0	58.2	911-934 bp	1.0	72.5
rps3-rps19_p4	TCTGAAACCGAGCGGATTTA	TTAAACTTCCGGGGACATGC	45.0	50.0	57.0	58.2	866-889 bp	1.0	71.9
rps3-rps19_p5	GTCTGAAACCGAGCGGATT	TTAAACTTCCGGGGACATGC	52.6	50.0	57.9	58.2	867-890 bp	1.0	71.2