Hotspot

rps8-rpl14

LSC · 85766-86165 · 400 bp

Back to Coulteria

Pi 0.0025

MarkerSeek score 51.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 400
variable_sites: 1
pi: 0.0025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GATGGTATTCGAACCGTCCC	GTTTCATTAGCACCCGAGGT	55.0	50.0	58.1	57.9	510-558 bp	1.0	78.2
rps8-rpl14_p2	GCAATGGTGTCCTTACCCAT	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.9	57.9	447-495 bp	1.0	78.1
rps8-rpl14_p3	GGTATTCGAACCGTCCCTTT	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.6	57.9	507-555 bp	1.0	76.1
rps8-rpl14_p4	GATGGTATTCGAACCGTCCC	AGTTTCATTAGCACCCGAGG	55.0	50.0	58.1	57.6	511-559 bp	1.0	75.8
rps8-rpl14_p5	GCAATGGTGTCCTTACCCAT	AGTTTCATTAGCACCCGAGG	50.0	50.0	57.9	57.6	448-496 bp	1.0	75.8