Hotspot

rps11-rpl36

LSC · 84387-84764 · 378 bp

Back to Coulteria

Pi 0.00277

MarkerSeek score 60.10

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 378
variable_sites: 1
pi: 0.00277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TACGCGCATTCTTACGTGAA	CGTAGACGCGGACGAATTAT	45.0	50.0	58.0	58.0	494-511 bp	1.0	82.5
rps11-rpl36_p2	TTACGCGCATTCTTACGTGA	CGTAGACGCGGACGAATTAT	45.0	50.0	58.0	58.0	495-512 bp	1.0	82.5
rps11-rpl36_p3	CCCTTTGGTATTTTACGCGC	CGTAGACGCGGACGAATTAT	50.0	50.0	57.8	58.0	507-524 bp	1.0	81.1
rps11-rpl36_p4	TACGCGCATTCTTACGTGAA	AAAATAAGGGCCTCCGTTCG	45.0	50.0	58.0	58.3	542-559 bp	1.0	80.4
rps11-rpl36_p5	TTACGCGCATTCTTACGTGA	AAAATAAGGGCCTCCGTTCG	45.0	50.0	58.0	58.3	543-560 bp	1.0	80.4