Hotspot

psbE-petL

LSC · 69247-70540 · 1294 bp

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Pi 0.000773

MarkerSeek score 59.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1294
variable_sites: 1
pi: 0.000773
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCG	45.0	55.0	58.0	58.2	1497 bp	1.0	78.5
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.9	58.2	1486 bp	1.0	78.3
psbE-petL_p3	TGACAAATAACCAACCCGCA	TCGTATCTTGCTCAGACCGA	45.0	50.0	58.0	58.3	1496 bp	1.0	77.9
psbE-petL_p4	CAACCCGCAATGAATAGGGA	TCGTATCTTGCTCAGACCGA	50.0	50.0	57.9	58.3	1485 bp	1.0	77.6
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.8	58.2	1409 bp	1.0	77.4