Hotspot

petA-psbJ

LSC · 67295-68478 · 1184 bp

Back to Coulteria

Pi 0.000845

MarkerSeek score 59.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1184
variable_sites: 1
pi: 0.000845
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1321 bp	1.0	75.2
petA-psbJ_p2	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1326 bp	1.0	74.7
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.4	57.6	1330 bp	1.0	74.2
petA-psbJ_p4	ACGTGTCCAAGGTCTCTTGT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.9	57.6	1322 bp	1.0	70.1
petA-psbJ_p5	ATCCGTTACGTGTCCAAGGT	ATTCCGGATTGGGTTCATCC	50.0	50.0	59.0	57.6	1329 bp	1.0	68.9