Hotspot

petN-psbM

LSC · 31150-32371 · 1222 bp

Back to Coulteria

Pi 0.000818

MarkerSeek score 59.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1222
variable_sites: 1
pi: 0.000818
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1384 bp	1.0	67.6
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1323 bp	1.0	66.0
petN-psbM_p3	CCCTCGTAGTATGGGGAAGA	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1333 bp	1.0	65.8
petN-psbM_p4	TCCCTCGTAGTATGGGGAAG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1334 bp	1.0	65.8
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1384 bp	1.0	63.4