Hotspot

trnC-GCA-petN

LSC · 29882-31059 · 1178 bp

Back to Coulteria

Pi 0.000849

MarkerSeek score 59.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1178
variable_sites: 1
pi: 0.000849
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1244 bp	1.0	78.2
trnC-GCA-petN_p2	GTATCATTTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	1296 bp	1.0	77.7
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1305 bp	1.0	76.6
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACGAGGG	50.0	55.0	58.1	57.6	1295 bp	1.0	76.4
trnC-GCA-petN_p5	GTATCATTTTGGCGGCATGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.9	57.6	1357 bp	1.0	76.1