Hotspot

atpH-atpI

LSC · 15003-16268 · 1266 bp

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Pi 0.00079

MarkerSeek score 59.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1266
variable_sites: 1
pi: 0.00079
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1353 bp	1.0	78.7
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1354 bp	1.0	77.5
atpH-atpI_p3	CCTTCTACAGCTTGACCTGC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.3	57.9	1434 bp	1.0	76.8
atpH-atpI_p4	TCCCTTCTACAGCTTGACCT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.7	57.9	1436 bp	1.0	76.4
atpH-atpI_p5	CCCTTCTACAGCTTGACCTG	TTTTGCAACTTTAGCTGCGG	55.0	45.0	57.6	57.9	1435 bp	1.0	75.8