Hotspot

trnK-UUU-rps16

LSC · 4360-5484 · 1125 bp

Back to Coulteria

Pi 0
MarkerSeek score 59.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1125
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC CCTTGAAAAAGGCGCTCAAC 55.0 50.0 57.9 58.2 1317-1322 bp 1.0 77.8
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC CTTGAAAAAGGCGCTCAACC 55.0 50.0 57.9 58.2 1316-1321 bp 1.0 77.8
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC AAGGCCGGTGTTTTTATGGA 55.0 45.0 57.9 57.7 1268-1273 bp 1.0 77.3
trnK-UUU-rps16_p4 CCGATGGTATGGACGAATCC AGGCCGGTGTTTTTATGGAA 55.0 45.0 57.9 57.7 1267-1272 bp 1.0 77.3
trnK-UUU-rps16_p5 CCGATGGTATGGACGAATCC TCCTTGAAAAAGGCGCTCAA 55.0 45.0 57.9 58.3 1318-1323 bp 1.0 77.2