Hotspot

rpl16

LSC · 85921-87403 · 1483 bp

Back to Couepia

Pi 0.00246

MarkerSeek score 57.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1483
variable_sites: 12
pi: 0.00246
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTGTTATTTCAATGCAAAGGGC	TCCTCTTTGTCTTCAATCAAAACA	39.1	33.3	58.2	57.0	1699-1707 bp	1.0	40.9
rpl16_p2	TCTGTTATTTCAATGCAAAGGGC	ACAAACTCTTTCATTCCTCTTTGT	39.1	33.3	58.2	57.0	1713-1721 bp	1.0	40.9
rpl16_p3	CTGTTATTTCAATGCAAAGGGC	TCCTCTTTGTCTTCAATCAAAACA	40.9	33.3	56.8	57.0	1698-1706 bp	1.0	40.9
rpl16_p4	TCTGTTATTTCAATGCAAAGGGC	ACTCTTTCATTCCTCTTTGTCT	39.1	36.4	58.2	55.0	1709-1717 bp	0.9	36.8
rpl16_p5	CTGTTATTTCAATGCAAAGGGC	ACTCTTTCATTCCTCTTTGTCT	40.9	36.4	56.8	55.0	1708-1716 bp	0.9	36.8