Hotspot

psbB

LSC · 76665-78191 · 1527 bp

Back to Couepia

Pi 0.000262

MarkerSeek score 44.00

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 2
pi: 0.000262
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	TCCCCGAGATAATCCACTGA	AGATTCAAAGAAAGGGTGGCA	50.0	42.9	57.2	57.8	1731 bp	1.0	64.1
psbB_p2	TCCCCGAGATAATCCACTGA	TCGTCGGTACCTTCTTTCAA	50.0	45.0	57.2	56.5	1695 bp	1.0	62.2
psbB_p3	TCCCCGAGATAATCCACTGAA	AGATTCAAAGAAAGGGTGGCA	47.6	42.9	58.0	57.8	1731 bp	1.0	61.9
psbB_p4	TTCCCCGAGATAATCCACTGA	AGATTCAAAGAAAGGGTGGCA	47.6	42.9	58.0	57.8	1732 bp	1.0	61.9
psbB_p5	TCCTTTCCCCGAGATAATCCA	AGATTCAAAGAAAGGGTGGCA	47.6	42.9	57.9	57.8	1736 bp	1.0	61.8