Hotspot

ndhD

SSC · 121760-123262 · 1503 bp

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Pi 0.00154

MarkerSeek score 43.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 10
pi: 0.00154
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGGATTCCTTTTTCATTTTCTGT	AGGGTTTTTCTGGTCCAAGT	30.4	45.0	54.7	56.9	1575 bp	1.0	40.5
ndhD_p2	AGGATTCCTTTTTCATTTTCTGT	AAGGGTTTTTCTGGTCCAAGT	30.4	42.9	54.7	57.6	1576 bp	1.0	40.5
ndhD_p3	AGGATTCCTTTTTCATTTTCTGT	AGGGTTTTTCTGGTCCAAGTT	30.4	42.9	54.7	57.6	1575 bp	1.0	40.5
ndhD_p4	AGGATTCCTTTTTCATTTTCTGTT	AGGGTTTTTCTGGTCCAAGT	29.2	45.0	55.4	56.9	1575 bp	1.0	40.5
ndhD_p5	GGATTCCTTTTTCATTTTCTGT	AGGGTTTTTCTGGTCCAAGT	31.8	45.0	53.2	56.9	1574 bp	1.0	40.5