Hotspot

rpl36-rps8

LSC · 84207-84743 · 537 bp

Back to Couepia

Pi 0.00319

MarkerSeek score 58.00

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 537
variable_sites: 7
pi: 0.00319
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	ATTCGTCCCCGTCTACGTAT	GCGGGATGGGGATTGTAATT	50.0	50.0	58.0	58.0	703-711 bp	1.0	81.3
rpl36-rps8_p2	ACTCTAATTCGTCCCCGTCT	GCGGGATGGGGATTGTAATT	50.0	50.0	57.9	58.0	709-717 bp	1.0	80.4
rpl36-rps8_p3	CAAATTTTACGAGCGGAGGC	GCGGGATGGGGATTGTAATT	50.0	50.0	57.8	58.0	667-675 bp	1.0	80.2
rpl36-rps8_p4	AATTCGTCCCCGTCTACGTA	GCGGGATGGGGATTGTAATT	50.0	50.0	58.2	58.0	704-712 bp	1.0	79.5
rpl36-rps8_p5	TTCGTCCCCGTCTACGTATT	GCGGGATGGGGATTGTAATT	50.0	50.0	58.2	58.0	702-710 bp	1.0	79.5