Hotspot

psbZ-trnG-UCC

LSC · 38162-38871 · 710 bp

Back to Couepia

Pi 0.00307

MarkerSeek score 59.40

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 9
pi: 0.00307
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	845-908 bp	1.0	81.1
psbZ-trnG-UCC_p2	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	841-904 bp	1.0	79.4
psbZ-trnG-UCC_p3	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	844-907 bp	1.0	78.3
psbZ-trnG-UCC_p4	TCTCCTGATGGTTGGTCGAG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.8	57.8	843-906 bp	1.0	74.7
psbZ-trnG-UCC_p5	CTTCTCCTGATGGTTGGTCG	CGGATAGCGGGAATCGAAC	55.0	57.9	58.0	58.2	867-930 bp	1.0	72.9