Hotspot

psbM-trnD-GUC

LSC · 30975-31988 · 1014 bp

Back to Couepia

Pi 0.00283

MarkerSeek score 59.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 9
pi: 0.00283
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACAAGAATGAACAGCGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.0	58.5	1114-1156 bp	1.0	78.1
psbM-trnD-GUC_p2	ATGAACAGCGCAGTAGCAAT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.3	58.5	1105-1147 bp	1.0	76.3
psbM-trnD-GUC_p3	TGAACAGCGCAGTAGCAATA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	1104-1146 bp	1.0	75.4
psbM-trnD-GUC_p4	ACAAGAATGAACAGCGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.4	58.5	1111-1153 bp	1.0	75.2
psbM-trnD-GUC_p5	AATGAACAGCGCAGTAGCAA	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.5	58.5	1106-1148 bp	1.0	74.6