Hotspot

atpH-atpI

LSC · 13831-15031 · 1201 bp

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Pi 0.00237

MarkerSeek score 57.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1201
variable_sites: 10
pi: 0.00237
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1287-1289 bp	1.0	79.3
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1279-1281 bp	1.0	79.1
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGG	45.0	55.0	58.0	58.2	1261-1263 bp	1.0	78.9
atpH-atpI_p4	AGCGGCAGAAATCAATGGAT	TAGGTGAATCCATGGAGGGG	45.0	55.0	57.9	58.2	1253-1255 bp	1.0	78.8
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1325-1327 bp	1.0	78.6