Hotspot

atpA-atpF

LSC · 11693-11759 · 67 bp

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Pi 0.0193

MarkerSeek score 58.90

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 3
pi: 0.0193
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	TACTAATCTCGTCGGCTCGA	TATTGGCATGTTTGGAGCGA	50.0	45.0	57.8	57.9	135-139 bp	1.0	80.7
atpA-atpF_p2	CCGTCGCCTACTTGAAGTAC	TATTGGCATGTTTGGAGCGA	55.0	45.0	58.1	57.9	217-221 bp	1.0	80.5
atpA-atpF_p3	GTCGCCTACTTGAAGTACGG	TATTGGCATGTTTGGAGCGA	55.0	45.0	58.1	57.9	215-219 bp	1.0	80.5
atpA-atpF_p4	TAATCTCGTCGGCTCGAATG	TATTGGCATGTTTGGAGCGA	50.0	45.0	57.6	57.9	132-136 bp	1.0	79.4
atpA-atpF_p5	ACTTGAAGTACGGTACCGGT	TATTGGCATGTTTGGAGCGA	50.0	45.0	58.4	57.9	208-212 bp	1.0	78.3