Hotspot

trnS-GCU-trnG-GCC

LSC · 7291-8254 · 964 bp

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Pi 0.00722
MarkerSeek score 58.30
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
964
variable_sites
20
pi
0.00722
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA GAATAGTTATTCCCCGAGGG 50.0 50.0 58.0 54.7 1036-1126 bp 1.0 58.3
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC GAATAGTTATTCCCCGAGGG 50.0 50.0 58.0 54.7 1035-1125 bp 1.0 58.3
trnS-GCU-trnG-GCC_p3 ATCAACGGAACCGGAAAGAG GAATAGTTATTCCCCGAGGG 50.0 50.0 57.9 54.7 1100-1190 bp 1.0 57.3
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 990-1080 bp 1.0 56.7
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 989-1079 bp 1.0 56.7