Hotspot

trnH-GUG-psbA

LSC · 112-509 · 398 bp

Back to Couepia

Pi 0.0176
MarkerSeek score 57.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
398
variable_sites
21
pi
0.0176
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CTGCCTTAATCCACTTGGCT CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 524-547 bp 1.0 80.5
trnH-GUG-psbA_p2 ACTGCCTTAATCCACTTGGC CCGTGCTAACCTTGGTATGG 50.0 55.0 58.2 58.3 525-548 bp 1.0 80.3
trnH-GUG-psbA_p3 CTGCCTTAATCCACTTGGCT ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 525-548 bp 1.0 80.0
trnH-GUG-psbA_p4 ACTGCCTTAATCCACTTGGC ACCGTGCTAACCTTGGTATG 50.0 50.0 58.2 57.6 526-549 bp 1.0 79.8
trnH-GUG-psbA_p5 AATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 50.0 55.0 57.7 58.3 517-540 bp 1.0 78.9