Hotspot

psbA

LSC · 368-1429 · 1062 bp

Back to Cotoneaster

Pi 0.000142

MarkerSeek score 32.90

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 4
pi: 0.000142
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ATTCTGGGTTATGGGCGAAC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.9	57.9	1768-1795 bp	0.985075	82.6
psbA_p2	ATTCTGGGTTATGGGCGAAC	CGGTATTGGTTGACATGGGT	50.0	50.0	57.9	57.9	1509-1534 bp	0.985075	82.5
psbA_p3	ATTCTGGGTTATGGGCGAAC	AGAATTCGTGTGCTTGGGAG	50.0	50.0	57.9	58.2	1419-1444 bp	0.985075	82.3
psbA_p4	ATTCTGGGTTATGGGCGAAC	AGAGAATTCGTGTGCTTGGG	50.0	50.0	57.9	58.2	1421-1446 bp	0.985075	82.3
psbA_p5	ATTCTGGGTTATGGGCGAAC	GAGAATTCGTGTGCTTGGGA	50.0	50.0	57.9	58.2	1420-1445 bp	0.985075	82.2