Hotspot

ndhF-rpl32

SSC · 116201-117246 · 1046 bp

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Pi 0.00634
MarkerSeek score 47.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1046
variable_sites
34
pi
0.00634
unique_haplotype_count
32
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCCGTTGGAAAAAGT TGAAATAGAGGTGCGCTTTT 40.0 40.0 57.0 55.7 1179-1265 bp 0.985075 56.9
ndhF-rpl32_p2 TTGTTGCCGTTGGAAAAAGT TTGAAATAGAGGTGCGCTTT 40.0 40.0 57.0 55.7 1180-1266 bp 0.985075 56.9
ndhF-rpl32_p3 TTGTTGCCGTTGGAAAAAGT TTTGAAATAGAGGTGCGCTT 40.0 40.0 57.0 55.7 1181-1267 bp 0.985075 56.9
ndhF-rpl32_p4 TTGTTGCCGTTGGAAAAAGT TTTTGAAATAGAGGTGCGCT 40.0 40.0 57.0 55.7 1182-1268 bp 0.985075 56.9
ndhF-rpl32_p5 TTGTTGCCGTTGGAAAAAGT TGAAATAGAGGTGCGCTTTTT 40.0 38.1 57.0 56.4 1179-1265 bp 0.985075 55.2