Hotspot

ndhC-trnV-UAC

LSC · 53828-54381 · 554 bp

Back to Cotoneaster

Pi 0.0097

MarkerSeek score 53.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 34
pi: 0.0097
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	741-852 bp	1.0	85.1
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	677-788 bp	1.0	85.1
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	AATGCACGTTGGGTCTTTGA	50.0	45.0	57.9	58.3	744-855 bp	1.0	83.9
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	ATGCACGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	743-854 bp	1.0	83.9
ndhC-trnV-UAC_p5	CTGGTCCTTTGCTAATCGGG	GCACGTTGGGTCTTTGAAAC	55.0	50.0	58.3	58.2	758-869 bp	1.0	83.4