Hotspot

psaA-ycf3

LSC · 44446-45147 · 702 bp

Back to Cotoneaster

Pi 0.00208

MarkerSeek score 49.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 8
pi: 0.00208
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TGGAAACAAGCAATAGCGCT	50.0	45.0	57.9	58.5	862-901 bp	0.985075	76.2
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	CAATAGCGCTTACTCCTGGT	50.0	50.0	57.9	57.4	852-891 bp	0.985075	75.1
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	GGAGGCTTGGTTCGATCAAG	50.0	55.0	57.9	58.6	893-932 bp	0.985075	74.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GCAATAGCGCTTACTCCTGG	50.0	55.0	57.9	58.8	853-892 bp	0.985075	73.7
psaA-ycf3_p5	TTGAGAAATGACCCGGTCTG	TGGAAACAAGCAATAGCGCT	50.0	45.0	57.5	58.5	882-921 bp	0.985075	73.3