Hotspot

rpoB-trnC-GCA

LSC · 27764-28975 · 1212 bp

Back to Cotoneaster

Pi 0.0042

MarkerSeek score 41.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1212
variable_sites: 25
pi: 0.0042
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TATTGCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1292-1339 bp	0.985075	77.8
rpoB-trnC-GCA_p2	TATTGCCTCATTTCCACCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.5	58.4	1274-1321 bp	0.985075	75.6
rpoB-trnC-GCA_p3	CATTTCCACCCCCAAGCAT	CCGGATTTGAACTGGGGAAA	52.6	50.0	58.0	58.1	1284-1331 bp	0.985075	73.4
rpoB-trnC-GCA_p4	ATATTGCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	47.6	50.0	58.0	58.1	1293-1340 bp	0.985075	73.1
rpoB-trnC-GCA_p5	TATTGCCTCATTTCCACCCC	TTTGTTGATCAGGCGACACC	50.0	50.0	57.5	58.8	1311-1358 bp	0.985075	72.2