Hotspot

trnR-UCU-atpA

LSC · 10110-10748 · 639 bp

Back to Cotoneaster

Pi 0.0159

MarkerSeek score 67.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 49
pi: 0.0159
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	627-887 bp	0.985075	72.8
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	626-886 bp	0.985075	72.8
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	47.6	50.0	57.3	58.4	566-826 bp	0.985075	72.3
trnR-UCU-atpA_p4	TGGATAGGACAGAGGTCTTCT	AGACATTTACCGACGAAGCG	47.6	50.0	56.9	58.4	601-861 bp	0.985075	69.2
trnR-UCU-atpA_p5	TGGAATGAAAAGCGTCCATT	AGACATTTACCGACGAAGCG	40.0	50.0	55.9	58.4	627-887 bp	0.985075	68.8