Hotspot

trnK-UUU-rps16

LSC · 4228-5189 · 962 bp

Back to Cotoneaster

Pi 0.00541
MarkerSeek score 55.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
962
variable_sites
23
pi
0.00541
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CGGAACTTCGCCCTAATCAA 50.0 50.0 58.2 58.0 1004-1060 bp 0.985075 83.0
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT CGGAACTTCGCCCTAATCAA 50.0 50.0 58.2 58.0 1003-1059 bp 0.985075 83.0
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA CGGAACTTCGCCCTAATCAA 50.0 50.0 58.3 58.0 1013-1069 bp 0.985075 82.0
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1059-1115 bp 0.985075 81.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1058-1114 bp 0.985075 81.3