Hotspot

rps8

LSC · 83346-83750 · 405 bp

Back to Cotinus

Pi 0.00941

MarkerSeek score 42.10

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 11
pi: 0.00941
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	TCTCCTACGGATCAGTCGAC	TCGCGATAGAGTGACAGTCT	55.0	50.0	58.0	58.0	1017-1125 bp	1.0	86.2
rps8_p2	GTGTCCTCTCCTACGGATCA	TCGCGATAGAGTGACAGTCT	55.0	50.0	57.9	58.0	1023-1131 bp	1.0	86.1
rps8_p3	TGTGTCCTCTCCTACGGATC	TCGCGATAGAGTGACAGTCT	55.0	50.0	57.9	58.0	1024-1132 bp	1.0	86.1
rps8_p4	GGCAAAACCCCCTTGAAGTA	TCGCGATAGAGTGACAGTCT	50.0	50.0	58.0	58.0	590-742 bp	0.857143	80.9
rps8_p5	GGCAAAACCCCCTTGAAGTA	GGGCCCGAGAATTAATGTGT	50.0	50.0	58.0	57.9	923-1075 bp	0.857143	80.0