Hotspot

rpl22

LSC · 86640-87080 · 441 bp

Back to Cotinus

Pi 0.015

MarkerSeek score 56.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 441
variable_sites: 20
pi: 0.015
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TTTGGCTGTGCAAACCAAAG	CCGGATCGAAATGTTGGCTA	45.0	50.0	58.0	58.1	1024-1432 bp	1.0	90.8
rpl22_p2	CGCCCTGCAATTTGTACTTG	GGAAGGGCTCCAATTGGTAG	50.0	55.0	58.0	57.9	1358-1766 bp	1.0	89.6
rpl22_p3	CGCCCTGCAATTTGTACTTG	CCGGATCGAAATGTTGGCTA	50.0	50.0	58.0	58.1	1444-1852 bp	1.0	89.4
rpl22_p4	TTTGGCTGTGCAAACCAAAG	TAGTTATGGGCTATGGGCGA	45.0	50.0	58.0	58.0	867-1064 bp	0.857143	86.0
rpl22_p5	CGCCCTGCAATTTGTACTTG	TAGTTATGGGCTATGGGCGA	50.0	50.0	58.0	58.0	1287-1484 bp	0.857143	84.7