Hotspot

rpl32-trnL-UAG

SSC · 117243-118394 · 1152 bp

Back to Cotinus

Pi 0.00948

MarkerSeek score 59.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1152
variable_sites: 28
pi: 0.00948
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACGGGGAATTCACAAAG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	877-1299 bp	0.714286	73.5
rpl32-trnL-UAG_p2	TCCACGGGGAATTCACAAAG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	878-1300 bp	0.714286	73.5
rpl32-trnL-UAG_p3	TCCACGGGGAATTCACAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	887-1309 bp	0.714286	72.5
rpl32-trnL-UAG_p4	TCCACGGGGAATTCACAAAG	TTATAAGATGCTCTGCCGCC	50.0	50.0	58.1	57.8	941-1363 bp	0.714286	72.1
rpl32-trnL-UAG_p5	TCCACGGGGAATTCACAAAG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.1	57.8	885-1307 bp	0.714286	72.0