Hotspot

rpl22-rps19

LSC · 87081-87113 · 33 bp

Back to Cotinus

Pi 0.108

MarkerSeek score 64.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 33
variable_sites: 11
pi: 0.108
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CTCATAAGAACGCCCACGAA	CCGGATCGAAATGTTGGCTA	50.0	50.0	58.0	58.1	526-993 bp	1.0	90.9
rpl22-rps19_p2	TAAGAACGCCCACGAATCTG	CCGGATCGAAATGTTGGCTA	50.0	50.0	58.0	58.1	522-989 bp	1.0	90.9
rpl22-rps19_p3	CTCATAAGAACGCCCACGAA	TAGTTATGGGCTATGGGCGA	50.0	50.0	58.0	58.0	434-625 bp	0.857143	86.1
rpl22-rps19_p4	TAAGAACGCCCACGAATCTG	TAGTTATGGGCTATGGGCGA	50.0	50.0	58.0	58.0	430-621 bp	0.857143	86.1
rpl22-rps19_p5	TTTGGCTGTGCAAACCAAAG	TAGTTATGGGCTATGGGCGA	45.0	50.0	58.0	58.0	867-1064 bp	0.857143	86.0