Hotspot

rps3-rpl22

LSC · 86602-86639 · 38 bp

Back to Cotinus

Pi 0.167

MarkerSeek score 61.80

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 38
variable_sites: 18
pi: 0.167
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TTTGGCTGTGCAAACCAAAG	TTCGTGGGCGTTCTTATGAG	45.0	50.0	58.0	58.0	453-518 bp	1.0	88.2
rps3-rpl22_p2	TTTGGCTGTGCAAACCAAAG	CAGATTCGTGGGCGTTCTTA	45.0	50.0	58.0	58.0	457-522 bp	1.0	88.1
rps3-rpl22_p3	CGCCCTGCAATTTGTACTTG	TTCGTGGGCGTTCTTATGAG	50.0	50.0	58.0	58.0	873-938 bp	1.0	85.1
rps3-rpl22_p4	CGCCCTGCAATTTGTACTTG	CAGATTCGTGGGCGTTCTTA	50.0	50.0	58.0	58.0	877-942 bp	1.0	85.0
rps3-rpl22_p5	CGCCCTGCAATTTGTACTTG	TAGTTATGGGCTATGGGCGA	50.0	50.0	58.0	58.0	1287-1484 bp	0.857143	84.7