Hotspot

rpl33-rps18

LSC · 70869-71077 · 209 bp

Back to Cotinus

Pi 0.0365

MarkerSeek score 69.30

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 209
variable_sites: 21
pi: 0.0365
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCTCGTCCCCCTCTTTTCTT	GCAGTAATTCGGCGAAATCG	50.0	50.0	58.0	58.0	1350-1541 bp	0.714286	73.6
rpl33-rps18_p2	AATGTGGCTAGGGGAAGAGT	GCAGTAATTCGGCGAAATCG	50.0	50.0	58.0	58.0	1191-1381 bp	0.714286	73.6
rpl33-rps18_p3	GGAAATGTGGCTAGGGGAAG	GCAGTAATTCGGCGAAATCG	55.0	50.0	57.9	58.0	1194-1384 bp	0.714286	73.3
rpl33-rps18_p4	GGTTCATGGCCAAGGGTAAA	GCAGTAATTCGGCGAAATCG	50.0	50.0	58.1	58.0	950-1140 bp	0.714286	73.3
rpl33-rps18_p5	AAAGGAGGTTCATGGCCAAG	GCAGTAATTCGGCGAAATCG	50.0	50.0	58.1	58.0	956-1146 bp	0.714286	73.3