Hotspot

psaA-ycf3

LSC · 44679-45386 · 708 bp

Back to Cotinus

Pi 0.00309

MarkerSeek score 60.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 7
pi: 0.00309
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TATCGCGCTTACTCCTGGTA	50.0	50.0	57.9	58.0	642-858 bp	1.0	83.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	CGCGCTTACTCCTGGTAATT	50.0	50.0	57.9	58.1	639-855 bp	1.0	83.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	612-828 bp	1.0	83.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	604-820 bp	1.0	82.8
psaA-ycf3_p5	TTGAGAAATGACCCGGTCTG	TATCGCGCTTACTCCTGGTA	50.0	50.0	57.5	58.0	662-878 bp	1.0	80.7