Hotspot

atpF-atpH

LSC · 14629-14894 · 266 bp

Back to Cotinus

Pi 0.0385

MarkerSeek score 82.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 266
variable_sites: 24
pi: 0.0385
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	440-462 bp	1.0	79.2
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GAGGCGGAGGGAAAAATACG	50.0	55.0	57.9	58.7	444-466 bp	1.0	79.1
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATACGAGG	50.0	55.0	57.9	58.7	441-463 bp	1.0	79.1
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCGGAGGGAAAAATACGAG	50.0	55.0	57.9	58.7	442-464 bp	1.0	79.1
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CGAGGCGGAGGGAAAAATAC	50.0	55.0	57.9	58.7	445-467 bp	1.0	79.0