Hotspot

atpA-atpF

LSC · 13262-13316 · 55 bp

Back to Cotinus

Pi 0.0416

MarkerSeek score 69.10

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 55
variable_sites: 5
pi: 0.0416
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATGCCATCGCCTACTTGAAG	TGGGGCGCTGAAAGAAATAA	50.0	45.0	58.0	57.8	198-201 bp	1.0	80.4
atpA-atpF_p2	ATGCCATCGCCTACTTGAAG	TTGGGGCGCTGAAAGAAATA	50.0	45.0	58.0	57.8	199-202 bp	1.0	80.4
atpA-atpF_p3	GATGCCATCGCCTACTTGAA	TGGGGCGCTGAAAGAAATAA	50.0	45.0	58.0	57.8	199-202 bp	1.0	80.4
atpA-atpF_p4	GATGCCATCGCCTACTTGAA	TTGGGGCGCTGAAAGAAATA	50.0	45.0	58.0	57.8	200-203 bp	1.0	80.3
atpA-atpF_p5	ATGCCATCGCCTACTTGAAG	GGGGCGCTGAAAGAAATAAC	50.0	50.0	58.0	57.4	197-200 bp	1.0	77.6