Hotspot

trnK-UUU-rps16

LSC · 4622-5709 · 1088 bp

Back to Cotinus

Pi 0.0118

MarkerSeek score 60.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1088
variable_sites: 40
pi: 0.0118
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.2	57.9	1179-1195 bp	1.0	81.9
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.2	57.9	1178-1194 bp	1.0	81.9
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.3	57.9	1188-1204 bp	1.0	80.9
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	AAGAAGGCGGGGGTTTTTAC	50.0	50.0	58.2	58.4	1172-1188 bp	1.0	80.0
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	AAGAAGGCGGGGGTTTTTAC	50.0	50.0	58.2	58.4	1171-1187 bp	1.0	80.0