Hotspot

trnH-GUG-psbA

LSC · 67-656 · 590 bp

Back to Cotinus

Pi 0.0162

MarkerSeek score 64.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 24
pi: 0.0162
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	628-667 bp	0.714286	73.4
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.2	57.9	629-668 bp	0.714286	73.2
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.7	57.9	621-660 bp	0.714286	71.9
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	677-716 bp	0.714286	71.1
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	678-717 bp	0.714286	70.9