Hotspot

ccsA-ndhD

SSC · 126519-127108 · 590 bp

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Pi 0.00311
MarkerSeek score 55.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
590
variable_sites
3
pi
0.00311
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 AGGGATAGGTCTACATAGTTATGGT CTCGCTATCAGTTGACAGGG 40.0 55.0 57.9 57.8 694-755 bp 1.0 44.3
ccsA-ndhD_p2 AGGGATAGGTCTACATAGTTATGGT GTTGACAGGGCCCAAGTTAT 40.0 50.0 57.9 57.8 684-745 bp 1.0 44.3
ccsA-ndhD_p3 AGGGATAGGTCTACATAGTTATGGT CCGATTTCGTTCTCTCGCTA 40.0 50.0 57.9 57.5 707-768 bp 1.0 44.3
ccsA-ndhD_p4 AGGGATAGGTCTACATAGTTATGGT ATCCCGATTTCGTTCTCTCG 40.0 50.0 57.9 57.5 710-771 bp 1.0 44.3
ccsA-ndhD_p5 GGGATAGGTCTACATAGTTATGGT CTCGCTATCAGTTGACAGGG 41.7 55.0 56.6 57.8 693-754 bp 1.0 44.3