Hotspot

rpl32-trnL-UAG

SSC · 124496-125371 · 876 bp

Back to Costus

Pi 0.00457
MarkerSeek score 47.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
876
variable_sites
6
pi
0.00457
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTTTTTACCGAGCGTTCAA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.0 58.0 931-969 bp 1.0 75.3
rpl32-trnL-UAG_p2 GGTTTTTACCGAGCGTTCAA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.0 58.0 932-970 bp 1.0 75.3
rpl32-trnL-UAG_p3 GGTTTTTACCGAGCGTTCAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.0 57.9 941-979 bp 1.0 74.3
rpl32-trnL-UAG_p4 ACCGAGCGTTCAAAAAGTTT AGCGTGTCTACCGATTTCAC 40.0 50.0 56.8 58.0 924-962 bp 1.0 73.9
rpl32-trnL-UAG_p5 ACCGAGCGTTCAAAAAGTTT CAGCGTGTCTACCGATTTCA 40.0 50.0 56.8 58.0 925-963 bp 1.0 73.9