Hotspot

psaJ-rpl33

LSC · 74457-75069 · 613 bp

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Pi 0
MarkerSeek score 44.10
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
613
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ATCGTTTATTCCCGGATGCC ACCCGGGGATTCCTTATTGA 50.0 50.0 58.1 58.1 694-741 bp 1.0 82.4
psaJ-rpl33_p2 GTCTTTAGCAGGCCTGTTGA ACCCGGGGATTCCTTATTGA 50.0 50.0 57.8 58.1 723-770 bp 1.0 81.7
psaJ-rpl33_p3 ATCGTTTATTCCCGGATGCC AGAAATACCCGGGGATTCCT 50.0 50.0 58.1 57.8 700-747 bp 1.0 81.5
psaJ-rpl33_p4 ATCGTTTATTCCCGGATGCC ACATCTTTACCCTTGGCCAC 50.0 50.0 58.1 57.8 627-674 bp 1.0 81.4
psaJ-rpl33_p5 ATCGTTTATTCCCGGATGCC CCCGGGGATTCCTTATTGAC 50.0 55.0 58.1 57.7 693-740 bp 1.0 81.0