Hotspot

petA-psbJ

LSC · 69692-70704 · 1013 bp

Back to Costus

Pi 0.00921
MarkerSeek score 55.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1013
variable_sites
17
pi
0.00921
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGGGTCCAAGGTCTTTTGTT TCTGGGTTGGGTTCATCTCT 50.0 50.0 58.3 58.0 1148 bp 1.0 78.3
petA-psbJ_p2 CGGGTCCAAGGTCTTTTGTT TGGGTTGGGTTCATCTCTCT 50.0 50.0 58.3 58.0 1146 bp 1.0 78.3
petA-psbJ_p3 CGGGTCCAAGGTCTTTTGTT TGGTATCCCTGTGATCGGTT 50.0 50.0 58.3 58.1 1197 bp 1.0 77.6
petA-psbJ_p4 TACGGGTCCAAGGTCTTTTG TCTGGGTTGGGTTCATCTCT 50.0 50.0 57.4 58.0 1150 bp 1.0 76.4
petA-psbJ_p5 TACGGGTCCAAGGTCTTTTG TGGGTTGGGTTCATCTCTCT 50.0 50.0 57.4 58.0 1148 bp 1.0 76.4