Hotspot

trnT-GGU-psbD

LSC · 36251-37496 · 1246 bp

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Pi 0.00575
MarkerSeek score 51.60
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1246
variable_sites
11
pi
0.00575
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1396-1473 bp 1.0 78.1
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1399-1476 bp 1.0 77.9
trnT-GGU-psbD_p3 AACGGGCCCTTTTAACTCAG ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1415-1492 bp 1.0 77.9
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG CAAAAACGAAACGGTCCCTC 55.0 50.0 58.1 57.6 1395-1472 bp 1.0 76.9
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA CAAAAACGAAACGGTCCCTC 50.0 50.0 58.1 57.6 1398-1475 bp 1.0 76.8