Hotspot

trnE-UUC-trnT-GGU

LSC · 35439-36178 · 740 bp

Back to Costus

Pi 0.00541
MarkerSeek score 44.20
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
740
variable_sites
6
pi
0.00541
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TCCTTTCGTAGTACCCCCAG GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 879 bp 1.0 79.9
trnE-UUC-trnT-GGU_p2 TCCTTTCGTAGTACCCCCAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.1 58.1 858 bp 1.0 79.5
trnE-UUC-trnT-GGU_p3 TCCTTTCGTAGTACCCCCAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.1 58.1 855 bp 1.0 79.3
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 843 bp 1.0 79.0
trnE-UUC-trnT-GGU_p5 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 822 bp 1.0 78.9