Hotspot

atpH-atpI

LSC · 16196-17427 · 1232 bp

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Pi 0.00433
MarkerSeek score 42.90
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1232
variable_sites
8
pi
0.00433
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 TACCTTCTACAGCTTGGCCT ATAGGGGAATCCATGGAGGG 50.0 55.0 58.0 57.9 1377-1494 bp 1.0 79.6
atpH-atpI_p2 TACCTTCTACAGCTTGGCCT ATTTTTGCAACCTTAGCCGC 50.0 45.0 58.0 57.9 1404-1521 bp 1.0 79.5
atpH-atpI_p3 AACAGAAGCGGCAGAAATCA ATAGGGGAATCCATGGAGGG 45.0 55.0 58.1 57.9 1292-1409 bp 1.0 79.2
atpH-atpI_p4 AACAGAAGCGGCAGAAATCA ATTTTTGCAACCTTAGCCGC 45.0 45.0 58.1 57.9 1319-1436 bp 1.0 79.1
atpH-atpI_p5 CAGCAGCAATAACAGAAGCG ATAGGGGAATCCATGGAGGG 50.0 55.0 57.8 57.9 1302-1419 bp 1.0 78.7