Hotspot

rpoB

LSC · 99364-103578 · 4215 bp

Back to Coscinodiscus

Pi 0.179

MarkerSeek score 73.90

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 4215
variable_sites: 744
pi: 0.179
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	GACGACGGCAGCTACTTTTA	CCAACCGATCTTACTCGTCG	50.0	55.0	58.0	58.2	1581 bp	0.333333	51.8
rpoB_p2	TTAATGACGACGGCAGCTAC	CCAACCGATCTTACTCGTCG	50.0	55.0	58.1	58.2	1586 bp	0.333333	51.3
rpoB_p3	CTTAATGACGACGGCAGCTA	CCAACCGATCTTACTCGTCG	50.0	55.0	57.8	58.2	1587 bp	0.333333	50.3
rpoB_p4	TTACCCTTAATGACGACGGC	CCAACCGATCTTACTCGTCG	50.0	55.0	57.7	58.2	1592 bp	0.333333	49.5
rpoB_p5	GACGACGGCAGCTACTTTTA	TCAGGAATAATGGTTGCCGT	50.0	45.0	58.0	57.2	1074 bp	0.333333	46.8