Hotspot

psbC

LSC · 81438-82853 · 1416 bp

Back to Coscinodiscus

Pi 0.107

MarkerSeek score 79.70

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1416
variable_sites: 152
pi: 0.107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	CCGATTTGTTCTGGGTTCGA	CAAGTTTTGGTGTTGCTGCA	50.0	45.0	58.2	58.0	2074-2075 bp	0.666667	73.4
psbC_p2	CTTCCCATTCTCCAGGTGTG	CAAGTTTTGGTGTTGCTGCA	55.0	45.0	57.9	58.0	2540 bp	0.333333	52.3
psbC_p3	CTTCCCATTCTCCAGGTGTG	GATGGTGATGCTGCAAACAC	55.0	50.0	57.9	58.0	2379 bp	0.333333	52.3
psbC_p4	CTTCCCATTCTCCAGGTGTG	TCCGTGCTTTTACACCAACA	55.0	45.0	57.9	58.0	2357 bp	0.333333	52.1
psbC_p5	CTTCCCATTCTCCAGGTGTG	CTTTTATGGGGTCCTGAGGC	55.0	55.0	57.9	57.9	2781 bp	0.333333	51.9