Hotspot

petA

LSC · 93869-94813 · 945 bp

Back to Coscinodiscus

Pi 0.139

MarkerSeek score 79.70

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 945
variable_sites: 131
pi: 0.139
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	TCAACATGCGCAGGTAAAGT	TTTAACCCCGTCAACAGACC	45.0	50.0	58.1	57.7	1305 bp	0.333333	50.4
petA_p2	CAGGTGCTTGTTCAACATGC	TTTAACCCCGTCAACAGACC	50.0	50.0	58.2	57.7	1316 bp	0.333333	49.5
petA_p3	GCAGGTGCTTGTTCAACATG	TTTAACCCCGTCAACAGACC	50.0	50.0	58.2	57.7	1317 bp	0.333333	49.5
petA_p4	TCAACATGCGCAGGTAAAGT	AACTTTCGCCCGGTGAATAT	45.0	45.0	58.1	57.6	1741 bp	0.333333	49.2
petA_p5	TCAACATGCGCAGGTAAAGT	ACTTTCGCCCGGTGAATATT	45.0	45.0	58.1	57.6	1740 bp	0.333333	49.2